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Exploring the molecular landscape of NNK-induced transformation: A comprehensive genome-wide CRISPR/Cas9 screening

Editorial: Structural understanding of the functional consequences of missense mutation

Unveiling immune checkpoint regulation : exploring the power of in vivo CRISPR screenings in cancer immunotherapy

Quantitative in vivo analyses reveal a complex pharmacogenomic landscape in lung adenocarcinoma.

The lack of knowledge about the relationship between tumor genotypes and therapeutic responses remains one of the most critical gaps in enabling the effective use of cancer therapies. Here we couple a multiplexed and quantitative experimental …

A functional taxonomy of tumor suppression in oncogenic KRAS-driven lung cancer

Cancer genotyping has identified a large number of putative tumor suppressor genes. Carcinogenesis is a multi-step process, however the importance and specific roles of many of these genes during tumor initiation, growth and progression remain …

Mapping the in vivo fitness landscape of lung adenocarcinoma tumor suppression in mice

The functional impact of most genomic alterations found in cancer, alone or in combination, remains largely unknown. Here we integrate tumor barcoding, CRISPR/Cas9-mediated genome editing and ultra-deep barcode sequencing to interrogate pairwise …

Multiplexed in vivo homology-directed repair and tumor barcoding enables parallel quantification of Kras variant oncogenicity

Large-scale genomic analyses of human cancers have cataloged somatic point mutations thought to initiate tumor development and sustain cancer growth. However, determining the functional significance of specific alterations remains a major bottleneck …

The Damaging Effect of Passenger Mutations on Cancer Progression.

Genomic instability and high mutation rates cause cancer to acquire numerous mutations and chromosomal alterations during its somatic evolution; most are termed passengers because they do not confer cancer phenotypes. Evolutionary simulations and …

A quantitative and multiplexed approach to uncover the fitness landscape of tumor suppression in vivo

A modified ziggurat algorithm for generating exponentially and normally distributed pseudorandom numbers

The ziggurat algorithm is a very fast rejection sampling method for generating pseudorandom numbers (PRNs) from statistical distributions. In the algorithm, rectangular sampling domains are layered on top of each other (resembling a ziggurat) to …